国产精品香港三级国产av-波多野结衣av高清中文字幕-亚洲日韩欧美一区久久久久我-美女扒开屁股让男人桶

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>CCL-1.3 L-M(TK-小鼠胸腺激酶缺陷細(xì)胞株

CCL-1.3 L-M(TK-小鼠胸腺激酶缺陷細(xì)胞株

簡要描述:CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株,
CCL-1.3 L-M(TK-小鼠胸腺激酶缺陷細(xì)胞株
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,
細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2225

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和i優(yōu)培養(yǎng)條件


CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株

ATCC® Number: CCL-1.3™    Price: $329.00
Designations: L-M(TK-) [LM(tk-), LMTK-]

Depositors: TR Chen

Biosafety Level:1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:fibroblast


Source:Tissue: subcutaneous connective tissue; areolar and adipose
Strain: C3H/An


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Tumorigenic:Yes

Antigen Expression:H-2k

Cytogenetic Analysis:modal number = 45; range = 42 to 48.

The stemline chromosome number is hyperdiploid with the 2S component occurring at 3.8%. Twenty-one markers were common to nearly all S metaphases studied. Of these, 13 were two-armed chromosomes, and eight, acrocentric. Four markers were isochromosomes by Robertsonian translocations from chromosomes 4, 12, 14 and 15. Normal X, 12, 14, 15 and 16 were absent. The X chromosome was present as an arm of Rb(X.3). (See Cancer Genet. Cytogenet. 24: 95-107, 1987 for a detailed karyotype description.)



Age: 100 days

Gender: male

Comments:The cells are 5-bromo-2'-deoxyuridine resistant (BUDR, BrdUrd) and thymidine kinase (TK) deficient.

HAT sensitive.

Tested and found negative for ectromelia virus (mousepox).



Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%


Subculturing: Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.


  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week


Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-1



References:868: Kit S, et al. Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridine. Exp. Cell Res. 31: 297-312, 1963. PubMed: 14056989

21410: Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673

25904: Hsu TC, Somers CE. Properties of L cells resistant to 5-bromodeoxyuridine. Exp. Cell Res. 26: 404-410, 1962. PubMed: 14449548

25907: Boone C, et al. Assignment of three human genes to chromosomes (LDH-A to 11, TK to 17, and IDH to 20) and evidence for translocation between human and mouse chromosomes in somatic cell hybrids (thymidine kinase-lactate dehydrogenase A-isocitrate dehydrogenase-C-11, E-17, and F-20 chromosomes). Proc. Natl. Acad. Sci. USA 69: 510-514, 1972. PubMed: 4110482

25910: Peterson JL, McBride OW. Cotransfer of linked eukaryotic genes and efficient transfer of hypoxanthine phosphoribosyltransferase by DNA-mediated gene transfer. Proc. Natl. Acad. Sci. USA 77: 1583-1587, 1980. PubMed: 6929511




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 日日躁夜夜躁狠狠躁超碰97| 国产欧美久久一区二区三区| 免费观看一区二区三区| 成人欧美一区二区三区| 人妻少妇av无码一区二区| 久久无码无码久久综合综合| 乱色欧美激惰| 亚洲午夜精品一区二区 | av性天堂网| 人人妻人人澡人人爽| 成人片黄网站色大片免费观看cn | 久久www免费人成人片| 欧美性xxxxx极品少妇直播| 免费久久人人爽人人爽av| 精品无码av无码免费专区| 中文字幕精品久久久久人妻| 国产成人精品一区二三区在线观看| 香蕉久久久久久av成人| 国产精品揄拍100视频| 奶头又大又白喷奶水av| 亚洲精品动漫免费二区| 无码熟妇人妻av在线影片| 99无码精品二区在线视频| 亚洲成aⅴ人片久青草影院| 麻豆国产96在线 | 日韩| 乱人伦中文无码视频| 免费人成视频x8x8入口app| 都市激情 在线 亚洲 国产| 边喂奶边中出的人妻| 一边捏奶头一边啪高潮视频| 99热精品久久只有精品| 中国丰满熟妇xxxx性| 国产成人综合亚洲看片| 无遮挡边吃摸边吃奶边做| 国产精品泄火熟女| 波多野结衣av高清中文字幕| 国产高清中文手机在线观看| 特级欧美aaaaaaa免费观看| 成 人 色综合| 人妻无码视频一区二区三区 | 欧洲熟妇色xxxxx欧美老妇伦|