国产精品香港三级国产av-波多野结衣av高清中文字幕-亚洲日韩欧美一区久久久久我-美女扒开屁股让男人桶

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-1720F9 小鼠畸胎瘤細(xì)胞

F9 小鼠畸胎瘤細(xì)胞

簡要描述:CRL-1720 F9 小鼠畸胎瘤細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種原代細(xì)胞、細(xì)胞系。細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1720
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:1632

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

CRL-1720 F9 小鼠畸胎瘤細(xì)胞


ATCC® Number:  CRL-1720™

Designations:  F9

Depositors:   S Strickland

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Mus musculus (mouse)

Morphology: epithelial

CRL-1720 F9 小鼠畸胎瘤細(xì)胞

Source: Organ: testis

Strain: 129

Disease: embryonal carcinoma; testicular teratoma

Cellular Products: plasminogen activator; laminin; type IV collagen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Roche FuGENE® Transfection Reagents)

Age:  embryo

Comments: F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cyclic AMP (cAMP).

Differentiating cells synthesize plasminogen activator, laminin and type IV collagen.

cAMP is active only on cells that have been treated with retinoic acid.

The cells maintain three copies of the beta 1 integrin gene.

Tested and found negative for ectromelia virus (mousepox).

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol: NOTE: culture vessels must be coated with 0.1% gelatin prior to use.To do so, cover the surface of the vessel with 0.1% gelatin (Difco) in sterile distilled water for 2 hours at 4C, then wash three times with sterile distilled water. Treated flasks and dishes can be stored at room temperature.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new coated culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 1160: Strickland S, et al. Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP. Cell 21: 347-355, 1980. PubMed: 6250719

1161: Strickland S, Mahdavi V. The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell 15: 393-403, 1978. PubMed: 214238

23426: Stephens LE, et al. Targeted deletion of beta 1 integrins in F9 embryonal carcinoma cells affects morphological differentiation but not tissue-specific gene expression. J. Cell Biol. 123: 1607-1620, 1993. PubMed: 7504677

26151: Berstine EG, et al. Alkaline phosphatase activity in mouse teratoma. Proc. Natl. Acad. Sci. USA 70: 3899-3903, 1973. PubMed: 4521215

32547: Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649



CRL-1720


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 最新亚洲人成无码网www电影| 国产熟人av一二三区| 久久久久久久亚洲av无码| 国产色在线 | 日韩| 国产精品视频全国免费观看| 欧美一夜爽爽爽爽爽爽| 人妻丰满熟妇无码区免费| 亚洲成aⅴ人片| 久久99精品久久久久久噜噜| 麻豆国产97在线 | 欧美| 少妇爆乳无码av无码波霸| 草草浮力地址线路①屁屁影院| 欧洲成人一区二区三区| 亚洲人成人网站在线观看| 亚洲中文无码av在线| 7777精品久久久大香线蕉| 奇米777四色影视在线看| 蜜桃视频插满18在线观看| 丰满老熟妇好大bbbbb| 中文无码人妻有码人妻中文字幕 | 性天堂av系列| 99精品国产在热久久婷婷| 国产男女猛视频在线观看| 人善交vide欧美| 久久午夜神器| 边啃奶头边躁狠狠躁3p| 比较有韵味的熟妇无码| 国产成人麻豆亚洲综合无码精品| 狠狠躁天天躁夜夜躁婷婷| 中文在线а天堂中文在线新版 | 吃奶呻吟打开双腿做受视频| 色偷偷噜噜噜亚洲男人| 国产在线国偷精品免费看| 中文午夜人妻无码看片| 欧美怡春院一区二区三区| 人妻洗澡被强公日日澡| 国产丝袜视频一区二区三区| 亚洲中文字幕无码爆乳av| 午夜视频久久久久一区| 综合久久国产九一剧情麻豆| 欧美精品黑人粗大视频|